![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K4785003
Designed by: Junfei Xia Group: iGEM23_SUSTech-CHINA (2023-10-08)
pTetR-LasR-luxR-GFP
The tetR promoter constitutively enhances expression of transcriptional factor LasR, which can bind to AHL 3OC12HSL. Then, LasR-3OC12HSL activator complex can open luxR promoter, leading to production of GFP which can be quantificationally measured by microplate reader.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 402
Illegal AgeI site found at 599 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1004
Illegal BsaI.rc site found at 1753
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Categories
Parameters
None |