Device

Part:BBa_K4785003

Designed by: Junfei Xia   Group: iGEM23_SUSTech-CHINA   (2023-10-08)


pTetR-LasR-luxR-GFP

The tetR promoter constitutively enhances expression of transcriptional factor LasR, which can bind to AHL 3OC12HSL. Then, LasR-3OC12HSL activator complex can open luxR promoter, leading to production of GFP which can be quantificationally measured by microplate reader.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 402
    Illegal AgeI site found at 599
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 1753


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